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Cellular Medicine
in Other Disorders
Health is determined by the function of millions
of cells composing the human body. The most frequent cause of
cellular malfunction is a chronic deficiency of essential nutrients
in the form of vitamins, amino acids, minerals, and trace elements.
The majority of the body’s proteins are composed
of collagen, elastin, and other connective tissue components.
Their production is regulated by vitamin C and the amino acids
lysine and proline, which the body does not produce.
Cellular Medicine identifies the optimum
daily intake of these and other essential nutrients needed for
health, and the application of this knowledge will reduce the
incidence of heart disease, cancer, degenerative diseases and
other serious health conditions.
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A
Nutrient Mixture Suppresses Carbon Tetrachloride Induced Hepatic
Toxicity in ICR Mice
- NEW -
M.W.
Roomi, V. Ivanov, A. Niedzwiecki, M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: FASEB, San Francisco, CA, April
1-5, 2006
Published in: The FASEB Journal, Abstract
85 |
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Liver
disease and toxicity is common, especially with many drug
treatments. Carbon tetrachloride (CT)-induced liver injury
in mice is an excellent model to evaluate the effectiveness
of agents on inhibition or reversal of liver damage. A unique
nutrient formulation consisting primarily of lysine, proline,
ascorbic acid and green tea extract (NM) has demonstrated
a broad spectrum of pharmacological, therapeutic, cardiovascular
and chemoprotective properties. We examined the effect of
a NM supplemented diet on liver and kidney tissue and serum
markers in ICR mice dosed with CT. Administration of CT
to mice on a regular diet resulted in marked increase in
serum AST and ALT levels (markers for liver). In contrast,
serum ALT and AST levels in NM-supplemented mice were of
low magnitude and comparable to control mice (not dosed
with CT). CT administration caused an intense degree of
liver necrosis that was less severe in the NM fed group.
These results are significant as they indicate that NM has
potential in treating and preventing liver disease. |
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Natural
Nutrient Mixture Effectively Reduces Collagen Matrix Contraction
Driven by Human Uterine Smooth Muscle Cells
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NEW -
V. Ivanov, M.W. Roomi,
T. Kalinovsky, A. Niedzwiecki, M.Rath
Published in: Journal of Obstetrics
and Gynaecology Research – 2006, 32(1):23-31 |
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Based
on these findings, we found that nutrients can effectively
counteract uterine myometrial contraction and MMP activity
in vitro, suggesting that pathophysiological mechanisms
of abnormal uterine myometrial contractility can be counteracted
by a combination of naturally occurring nutrients. These
mechanisms might involve extracellular matrix remodeling. |
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A
Nutrient Mixture Attenuates Acetaminophen Hepatic and Renal
Toxicity in ICR - NEW -
M.W. Roomi, V. Ivanov, A. Niedzwiecki,
M. Rath
Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara,
CA 95050
Presented at: Society of Toxicology, San
Diego, CA, March 5-9, 2006
Published in: The Toxicologists (suppl
Toxicological Sciences) 90 (1), Abstract 1398, pg 285, 2006. |
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Overdosing
with acetaminophen, a common over-the-counter analgesic,
is often fatal, causing severe liver and kidney damage in
humans and animals. We tested the protective effect of a
nutrient supplemented diet (0.5% NM) in mice injected ip
with acetaminophen (600 mg/kg) using a nutrient mixture
that has previously demonstrated therapeutic and chemoprotective
properties. The nutrient supplemented mice were protected
against acetaminophen-induced liver and kidney damage, while
the unsupplemented mice exhibited liver and kidney pathology
and associated serum enzyme abnormality. Thus, the nutrient
mixture provides protection against acetaminophen-induced
hepatic and renal damage. |
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Suppression
of Human Rheumatoid Synovial Fibroblast MMP-2 Expression and
Marix Invasion by Nutrient Synergy (2004)
M.W.
Roomi, V. Ivanov, A. Niedzwiecki, M. Rath
Matthias Rath Research, Cancer Research Division, Santa Clara,
CA 95050
Presented at: 9th World Congress of the
Osteoarthritis Research Society International, Chicago, Illinois,
December 2-5, 2004.
Published in: Osteoarthritis and Cartilage,
Abstract #193. |
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In
patients with rheumatoid arthritis and other inflammatory
diseases, MMPs are expressed in synovial membranes and have
been implicated in synovial tissue invasion into adjacent
cartilage and bone. Standard anti- arthritic drugs, used for
prolonged periods, have been associated with serious side
effects. We studied the in vitro effect of a mixture of nutrients
containing ascorbic acid, lysine, arginine and epigallocatechin
gallate on human synovial fibroblast growth, MMP expression,
and invasive potential. Our results demonstrated complete
inhibition of MMP-2 expression and invasive potential of synovial
fibroblasts at 500 µg/ml, suggesting this nutrient mixture
has excellent potential as a safe anti-inflammatory agent. |
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Natural
Nutrient Mixture of Ascorbic Acid with Amino Acids Lysine,
Arginine, Cysteine and Proline, and Green Tea Polyphenols
Effectively Reduces Collagen Matrix Contraction Driven by
Human Uterine Smooth Muscle Cells
V.Ivanov,
S. Ivanova, M. W. Roomi, A. Niedzwiecki, M. Rath
Matthias Rath BV, Research Institute, Santa Clara, CA, USA
Presented at: 12th International Congress
of Endocrinology, Lisbon, Portugal, August 31-September 4,
2004.
Published in: Conference proceedings,
Abstract #1166, pg 360.
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Disturbances
in hormone regulation and inflammation-related processes have
been shown to play roles in abnormal uterine contractility,
which causes preterm delivery and can lead to perinatal morbidity
and mortality. We used an in vitro model of collagen I gel
contraction driven by embedded cultured human uterine smooth
muscle cells to study the effect of various nutrients on uterine
contraction. Among the bioflavonoids and plant extracts tested,
we found that green tea leaf extract and epigallocatechin
gallate showed the strongest ability to counteract uterine
smooth muscle cell contraction. Furthermore, addition of ascorbic
acid and amino acids lysine, arginine, cysteine and proline
to green tea extract further increased its effectiveness.
A reduction in gel contraction correlated with decreased MMP.
The results of this study are significant since they demonstrate
the great potential of nutrients in preventing preterm delivery
and its sequelae. |
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Down
Regulation of MMP-2 Expression and Invasion Potential of Human
Gingival Fibroblasts
M.W. Roomi,
V. Ivanov, A. Niedzwiecki, M. Rath
Matthias Rath Research., Cancer Research Division, Santa Clara,
CA 95050
Presented at: XXVIII Scientific Meeting
of the South African Division of the International Association
for Dental Research, Pretoria, South Africa, September 2-3,
2004
Published in: Conference proceedings,
Abstract #37, pg 42 |
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Gingivitis,
the earliest stage of gum disease, affects three out of four
adults over the age of 35. Left untreated, it progresses to
periodontitis, an advanced stage of gum disease characterized
by additional destruction of gum tissue and progression to
the bone. MMPs play a critical role in this process as, in
concert, they degrade the majority of the extracellular matrix
components. We studied the effect of a combination (NM) of
ascorbic acid, lysine, proline, and green tea extract on expression
of MMPs by gingival fibroblasts in cell culture. NM significantly
inhibited MMP-2 expression and invasion through Matrigel,
suggesting NM has great potential as a therapeutic tool in
prevention and treatment of gingivitis and its sequelae. |
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| Allergy |
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Nutrient
Mixture Prevents Hyper-Reactive Response Of Bronchial Muscle
To Inflammatory And Allergic Stimuli.
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NEW -
Ivanov V, Roomi MW, Niedzwiecki
A, Rath M
Matthias Rath Research Institute BV, Santa Clara, CA.
Presented at: 46th Annual Meeting of the
American College of Nutrition, Charleston, South Carolina,
Sept 22-25, 2005.
Published in: Journal of the American
College of Nutrition, vol 24(5), Oct 2005, abstract #74
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Asthma,
a chronic pathological condition manifested by bronchial hyper-reactivity
and bronchial spasm is poorly managed by conventional treatment.
Pathogenesis is linked to infection, inflammation, and allergic
reaction to pollution. We found that a nutrient mixture of
ascorbic acid, amino acids lysine, proline, arginine and cysteine,
and polyphenols from green tea extract significantly inhibited
the hyper-reactive response of bronchial smooth muscle cells
in vitro to histamine and to inflammatory mediators, such
as bacterial lipopolysaccharides and endothelin-1. Furthermore,
the relaxation effect of the nutrient mixture was greater
than the combined effect of its individual components. These
findings are significant as they indicate that nutrient synergy
has therapeutic potential in safely and effectively controlling
asthma. |
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| Bone
and Cartilage |
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Matrix
Metalloproteinase-2 Inhibition and Invasion Potential in Human
Chondrocytes by Nutrient Synergy – A Specific Mixture
of Nutrients Containing Lysine, Proline, Ascorbic Acid and
Epigallocatechin Gallate (2002)
Roomi
MW, Ivanov V, Netke SP, Niedzwiecki A, Rath M.
Presented at: American Association of
Cancer Research Special Conference in Cancer Research: Proteases,
Extracellular Matrix and Cancer, Hilton Head Island, South
Carolina, Oct. 9-13, 2002.
Published in: Conference Proceedings,
Abstract B36.
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The
processes of cartilage erosion and synovial inflammation associated
with osteoarthritis are thought to result from increased MMP
expression by chondrocytes. The synergistic inhibitory effects
of the nutrient mixture of lysine, proline, ascorbic acid
and epigallocatechin gallate (from green tea extract) on human
chondrocyte MMP-2 expression and matrix invasion without cell
toxicity demonstrated in this study suggest that this nutrient
combination has potential as an anti-inflammatory agent for
safe, natural therapeutic use in osteoarthritis. |
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| Infectious
Disease |
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How
Micronutrients Can Control HIV/AIDS
Matthias
Rath, M.D. and Aleksandra Niedzwiecki, Ph.D.
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In
Khayelitsha, a suburb of Cape Town, South Africa, the Dr.
Health Foundation conducted a nutritional program for HIV-positive
patients with advanced AIDS and who were not taking any ARV
drugs. The goal of the program was to show that a combination
of vitamins and other micronutrients can improve immune system
function even among patients in the advanced stages of AIDS.
Blood tests performed at the start, and after one and two
months on the nutrient program, showed that markers immune
system health significantly increased. Patients that were
in the most advanced stages of AIDS enjoyed the highest levels
of improvement of immune functions. The vitamins effectively
reversed the course of AIDS in the patients.
The science behind this study are featured in the PowerPoint
presentation “How micronutrients work in controlling
HIV/AIDS and viral diseases.” Download the presentation
here.
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| Metabolic
Activities of Nutrient Synergy |
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Serum
Markers of the Liver, Heart, and Kidney and Lipid Profile
and Histopathology in ODS Rats Treated With Nutrient Synergy
(2003)
Roomi MW, Ivanov V, Netke
SP, Niedzwiecki A, Rath M.
Presented at: American College of Nutrition,
Nashville, Tennessee, October 9-12, 2003.
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Nutrient
Synergy has been shown in previous studies to have potent
anti-metastatic activity. To address safety issues, our research
team studied the effect of feeding different levels of this
nutrient mixture for seven days on the clinical chemistry
of adult female ODS rats, such as functional serum enzymes
of the liver, heart, and kidney, as well as lipid profile
and histopathology. Study results demonstrate that not only
is Nutrient Synergy safe at higher doses, but also is associated
with improved cellular functions in various organs in the
body. |
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